Measurement and Manipulation of Intracellular Ions

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Academic Press, 10.10.1995 - 400 Seiten
This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures.
  • A one-source reference for measuring and manipulating intracellular ions and for comparing and evaluating current methodologies
  • Includes overviews of Optical probes and reagents
  • Fabrication and use of ion-selective microelectrodes
  • Use of NMR spectroscopy
  • Ionophores
 

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Inhalt

Chapter 1 LightOpticalBased Reagents for the Measurement and Manipulation of Ions Metabolites and Macromolecules in Living Cells
1
Chapter 2 Use of IonSelective Microelectrodes for Recording Intracellular Ion Levels
17
Chapter 3 Use of NMR Spectroscopy to Measure Intracellular Ion Concentrations
39
Chapter 4 Use of Ionophores for Manipulating Intracellular Ion Concentrations
52
An Introduction to Basic Experimental Principles
69
Combined Photometry with Electrophysiology
81
Chapter 7 Use of Chelators and Photoactivatable Caged Compounds to Manipulate Cytosolic Calcium
123
Chapter 8 Measurement of Cytosolic Calcium by 19F NMR
153
Chapter 12 Manipulation and Regulation of Cytosolic pH
252
Chapter 13 Fluorescence Measurements of Cytosolic Sodium Concentration
274
Chapter 14 Measurement of Cytosolic Sodium Using IonSelective Microelectrodes
289
Chapter 15 A Practical Guide to the Use of Fluorescent Indicators for the Measurement of Cytosolic Free Magnesium
304
Chapter 16 Measurement of Cytosolic Magnesium by 31P NMR Spectroscopy
319
Chapter 17 ChlorideSensitive Fluorescent Indicators
328
Chapter 18 Measurement of Cytosolic Chloride Activity by IonSelective Microelectrodes
340
Chapter 19 Use of IonSelective Microelectrodes and Fluorescent Probes to Measure Cell Volume
361

Chapter 9 Fluorescence Measurement of Cytosolic pH in Cultured Rodent Astrocytes
196
Chapter 10 Brain Cell AcidBase Changes Measured with IonSelective Microelectrodes
214
Chapter 11 Measurement of Cytosolic pH by Nuclear Magnetic Resonance Spectroscopy
233

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Über den Autor (1995)

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.

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