Principles and Techniques of Biochemistry and Molecular BiologyKeith Wilson, John Walker Cambridge University Press, 04.03.2010 This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained. |
Inhalt
Basic principles | 1 |
Cell culture techniques | 38 |
Centrifugation | 73 |
Molecular biology bioinformatics and basic techniques | 138 |
Recombinant DNA and genetic analysis | 195 |
Immunochemical techniques | 263 |
Protein structure purification characterisation | 300 |
Mass spectrometric techniques | 352 |
I Spectrophotometric techniques | 477 |
II Structure and interactions | 522 |
Radioisotope techniques | 553 |
Principles of clinical biochemistry | 625 |
Cell membrane receptors and cell signalling | 660 |
Drug discovery and development | 709 |
736 | |
Electrophoretic techniques | 399 |
Andere Ausgaben - Alle anzeigen
Principles and Techniques of Biochemistry and Molecular Biology Keith Wilson,John Walker Eingeschränkte Leseprobe - 2010 |
Principles and Techniques of Biochemistry and Molecular Biology Keith Wilson,John Walker Keine Leseprobe verfügbar - 2010 |
Häufige Begriffe und Wortgruppen
activity agonist allows amino acid amount analysis analyte antibody applications assay binding biological buffer calculated carried cause cell culture cells centrifugation charged chromatography cloning column complex components concentration constant containing culture detection determined drug effect electron energy enzyme example expressed factor field fluorescence fragments function further gene give glucose groups important increase interaction involved ions known labelled levels ligand light mass mean measure medium membrane method microscope molecular molecules occur organic particles particular peptide phase plate positive possible preparation present probe produce protein range reaction receptor referred region relative removed residues response result sample selected separation sequence signal single solution specific standard structure studies substrate surface Table techniques termed tissue types units usually vector