Engineering the Genetic Code: Expanding the Amino Acid Repertoire for the Design of Novel ProteinsJohn Wiley & Sons, 12.05.2006 - 312 Seiten The ability to introduce non-canonical amino acids in vivo has greatly expanded the repertoire of accessible proteins for basic research and biotechnological application. Here, the different methods and strategies to incorporate new or modified amino acids are explained in detail, including a lot of practical advice for first-time users of this powerful technique. Novel applications in protein biochemistry, genomics, biotechnology and biomedicine made possible by the expansion of the genetic code are discussed and numerous examples are given. Essential reading for all molecular life scientists who want to stay ahead in their research. |
Inhalt
| 1 | |
2 A Brief History of an Expanded Amino Acid Repertoire | 13 |
3 Basic Features of the Cellular Translation Apparatus | 31 |
4 Amino Acids and Codons Code Organization and Protein Structure | 66 |
5 Reprograming the Cellular Translation Machinery | 90 |
6 Implications and Insights From Reprogrammed Translation and Code Evolution to Artificial Life | 184 |
7 Some Practical Potentials of Reprogrammed Cellular Translation | 213 |
Epilogue | 261 |
| 263 | |
Andere Ausgaben - Alle anzeigen
Engineering the Genetic Code: Expanding the Amino Acid Repertoire for the ... Nediljko Budisa Keine Leseprobe verfügbar - 2006 |
Engineering the Genetic Code: Expanding the Amino Acid Repertoire for the ... Nediljko Budisa Keine Leseprobe verfügbar - 2005 |
Engineering the Genetic Code: Expanding the Amino Acid Repertoire for the ... Nediljko Budisa Keine Leseprobe verfügbar - 2006 |
Häufige Begriffe und Wortgruppen
AARS Academy of Sciences active American Chemical Society amino acid analogs amino acid repertoire Aminoacyl-tRNA Synthetases aminoacylation analogs and surrogates annexin-V anticodon auxotrophic Biochemical Biochemistry biosynthesis Budisa canonical amino acids catalytic cellular chromophore code engineering coding triplets codon reassignment cognate context coworkers editing efficient encoded enzyme Escherichia coli eukaryotic evolution example experimental fluorinated folding function gene genetic code genome green fluorescent protein Huber hydrophobic Ibba interactions intracellular Journal of Molecular Landes Bioscience ligation living cells mechanisms metabolic Molecular Biology molecules Moroder mRNA mutant National Academy natural noncanonical amino acids norleucine nucleotides ofthe organisms pathways peptide polypeptide posttranslational modifications properties protein engineering protein sequences protein structure protein synthesis protein translation proteome reaction replacement residues ribosome Schultz Section SeMet side-chains substitution substrate specificity suppression suppressor tRNA synthetic target protein tion Tirrell toxic tryptophan TyrRS unnatural amino acids vitro vivo yeast