Effect of E65 and E70 splice isoforms on electrophysiological properties of Kv10.1
Project Report from the year 2013 in the subject Biology - Diseases, Health, Nutrition, grade: 1.3, University of Göttingen (Max Plank Institute for Experimental Medicine), language: English, abstract: Kv10.1, the voltage-gated non-inactivating delayed rectifier potassium channel, is overexpressed in variety of cancer cells and is involved in oncogenesis and tumor progression. Its splice variants E65 and E70, which were discovered in melanoma cell lines, have no conducting abilities but may physically interact with Kv10.1 and thereby regulate its function. Here, we investigated possible influence of E65 and E70 on electrophysiological properties of Kv10.1. Analysis of current-voltage relationships revealed a dose-dependent reduction of Kv10.1 current mediated by both splice variants. The channel demonstrated characteristic feature of activation kinetics, a Cole-Moore shift, irrespective of the isoforms presence. Both E65 and E70 were able to increase the rise time after -60 mV conditioning when expressed at 1:10 ratio with full length channel. Co-expression of E65 or E70 with Kv1.4 did not resulted in considerable changes in channel activity; therefore interactions of splice variants with Kv10.1 are likely to be specific. Downregulation of Kv10.1 activity by E65 and E70 splice variants may modulate tumorigenesis and be associated with less aggressive forms of cancer.
Was andere dazu sagen - Rezension schreiben
Es wurden keine Rezensionen gefunden.
Andere Ausgaben - Alle anzeigen
1:10 proportion Activation of Kv10.1 Alternative splicing cell surface cKNA co-expressed with full-length Co-expression of E65 co-injected current magnitude current-voltage relationships Data are represented depolarizing pulses Downregulation E70 isoform E70 splice isoforms E70 splice variants eBook and book Effect of E65 effect on Kv1.4 electrophysiological properties elicited endocytosis ethanol exons expression of Kv10.1 FitMaster full length channel gentamicin HEKA Electronics holding potential I-V protocol immunoblotting inactivation influence of E65 interact with Kv10.1 interactions between Kv10.1 ion channels Kohl Kv10.1 activation kinetics Kv10.1 channel requires Kv10.1 current leak subtraction linearized mRNA Napp ng/pal ng/ul oocytes expressing Pardo L.A. potassium channel prepulse potentials presence of E65 properties of Kv10.1 protein publish your bachelor's Ramos Gomes F ratio reduction of Kv10.1 represented as means sale Upload sample slight inward rectification surface expression TEVC method text at www.GRIN.com theophylline Upload your text Voltage mv voltage-gated www.GRIN.com and publish Xenopus laevis Xenopus oocytes