Gold and Silver Staining: Techniques in Molecular MorphologyGerhard W. Hacker, Jiang Gu CRC Press, 17.04.2002 - 264 Seiten Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know a |
Inhalt
1 Silver Stains for the Identification of Neuroendocrine Cell Types | 1 |
2 Autometallographic Tracing of Gold Silver Bismuth Mercury and Zinc | 13 |
3 Silver and GoldBased Autometallography of Nanogold174 | 29 |
4 Immunogold8211Silver Staining for Light Microscopy Using Colloidal or Clustered Gold Nanogold174 | 47 |
5 The Protein A8211Gold8211Silver Staining Method | 71 |
6 Microscopic Uses of Nanogold174 | 85 |
7 Combined Fluorescent and Gold Probes for Microscopic and Morphological Investigations | 107 |
8 Detection of Human Papillo mavirus by In Situ Hybridization Using an In Situ PCR Thermal Cycler | 119 |
Applications in Cell and Molecular Biology | 145 |
11 PreEmbedding Immunoelectron Microscopy with Nanogold 174Immunolabeling Silver Enhancement and Its Stabilization by Gold | 169 |
Light and Electron Microscopy | 177 |
13 Highly Sensitive Ultrastructural Immunogold Detection Using Tyramide and Gold Enhancement | 189 |
14 In Situ Hybridization at the Electron Microscopic Level | 199 |
15 Immunogold8211Silver Staining for Scanning Electron Microscopy in Cancer Research | 211 |
16 Immunogold8211Silver Scanning Electron Microscopy Using Glycerol Liquid Substitution | 223 |
| 235 | |
The Contribution of Autometallography and Catalyzed Reporter Deposition to the Rejuvenation of In Situ Hybridization | 127 |
Andere Ausgaben - Alle anzeigen
Gold and Silver Staining: Techniques in Molecular Morphology Gerhard W. Hacker,Jiang Gu Keine Leseprobe verfügbar - 2002 |
Gold and Silver Staining: Techniques in Molecular Morphology Gerhard W. Hacker,Jiang Gu Keine Leseprobe verfügbar - 2002 |
Häufige Begriffe und Wortgruppen
acid amplification anti antigens applications autometallography cell surface changes of PBS chloride citrate colloidal gold colloidal gold particles conjugate counterstained covalently coverslips CRC Press Cytochem Danscher deionized detection diluted distilled water EM-ISH embedding epitope ethanol Figure fixation fluorescence G.W. Hacker glutaraldehyde glycerol gold enhancement gold labeling gold–silver Grimelius Hainfeld Hauser-Kronberger Histochem Histochemistry hybridization IGSS immuno immunocytochemistry immunogold labeling immunogold–silver staining immunohistochemistry immunolabeling immunostaining Incubate J.M. Polak light and electron light microscope marker membrane ment mercury Microsc molecular molecules Nanogold Nanogold particles Nanogold-labeled Nanoprobes neurons PAGS staining peptide postembedding pre-embedding primary antibody protein Protocol resin Rinse room temperature samples secondary antibody sensitivity serum signal silver acetate silver enhancement silver stains slides sodium chloride sodium thiosulfate solution specimens staining method streptavidin tech technique tion tissue sections tyramide ultrastructural ultrathin visualization zinc