Microbial Cell Factories Engineering for Production of Biomolecules

Vijai Singh
Academic Press, 13.02.2021 - 488 Seiten

Microbial Cell Factories Engineering for Production of Biomolecules presents a compilation of chapters written by eminent scientists worldwide. Sections cover major tools and technologies for DNA synthesis, design of biosynthetic pathways, synthetic biology tools, biosensors, cell-free systems, computer-aided design, OMICS tools, CRISPR/Cas systems, and many more. Although it is not easy to find relevant information collated in a single volume, the book covers the production of a wide range of biomolecules from several MCFs, including Escherichia coli, Bacillus subtilis, Pseudomonas putida, Streptomyces, Corynebacterium, Cyanobacteria, Saccharomyces cerevisiae, Pichia pastoris and Yarrowia lipolytica, and algae, among many others.

This will be an excellent platform from which scientific knowledge can grow and widen in MCF engineering research for the production of biomolecules. Needless to say, the book is a valuable source of information not only for researchers designing cell factories, but also for students, metabolic engineers, synthetic biologists, genome engineers, industrialists, stakeholders and policymakers interested in harnessing the potential of MCFs in several fields.

  • Offers basic understanding and a clear picture of various MCFs
  • Explains several tools and technologies, including DNA synthesis, synthetic biology tools, genome editing, biosensors, computer-aided design, and OMICS tools, among others
  • Harnesses the potential of engineered MCFs to produce a wide range of biomolecules for industrial, therapeutic, pharmaceutical, nutraceutical and biotechnological applications
  • Highlights the advances, challenges, and future opportunities in designing MCFs


An introduction to microbial cell factories for production of biomolecules
Advances in long DNA synthesis
Discovery of enzymes responsible for cyclization and postmodification in triterpenoid biosynthesis
Synthetic biology approaches for secondary metabolism engineering
Synthetic biology design tools for metabolic engineering
Metabolic engineering for microbial cell factories
CRISPRbased tools for microbial cell factories
Escherichia coli the workhorse cell factory for the production of chemicals
Cyanobacteriabased microbial cell factories for production of industrial products
Integrated omics perspective to understand the production of highvalue added biomolecules HVABs in microal
Saccharomyces cerevisiae as a microbial cell factory
Pichia pastorisbased microbial cell factories
Yarrowia lipolytica engineering as a source of microbial cell factories
Engineering of microbial cell factories for production of plantbased natural products
Engineering of microbial cell factories for omicsguided production of medically important biomolecules
Advances and applications of cellfree systems for metabolic production

Bacillus subtilisbased microbial cell factories
Pseudomonas putidabased cell factories
Streptomycesbased cell factories for production of biomolecules and bioactive metabolites
Corynebacterium glutamicum as a robust microbial factory for production of valueadded proteins and small mol
Production of high valueadded chemicals by engineering methylotrophic cell factories
Microbial biosensors for discovery and engineering of enzymes and metabolism
Manipulation of global regulators in Escherichia coli for the synthesis of biotechnologically relevant products
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Autoren-Profil (2021)

Dr Vijai Singh is an Associate Professor and Dean (Research & Innovation) at School of Sciences, Indrashil University, Rajpur, Mehsana, Gujarat, India. He was an Assistant Professor in the Department of Biological Sciences and Biotechnology at the Institute of Advanced Research, Gandhinagar, India and also an Assistant Professor in the Department of Biotechnology at the Invertis University, Bareilly, India. Prior to that, he was a Postdoctoral Fellow in the Synthetic Biology Group at the Institute of Systems and Synthetic Biology, Paris, France and School of Energy & Chemical Engineering at the Ulsan National Institute of Science and Technology, Ulsan, South Korea. He received his Ph.D. in Biotechnology (2009) from the National Bureau of Fish Genetic Resources, Uttar Pradesh Technical University, Lucknow, India with a research focus on the development of molecular and immunoassays for diagnosis of Aeromonas hydrophila. His research interests are focused on building novel biosynthetic pathways for production of medically and industrially important biomolecules. Additionally, his laboratory is working on CRISPR-Cas9 tools for genome editing. He has more than 8 years of research and teaching experience in synthetic biology, metabolic engineering, bioinformatics, microbiology, and industrial microbiology. He has published 79 articles, 51 chapters, 15 books and 4 patents. He serves as an associate editor, editorial board member, and reviewer of several peer-reviewed journals. He is also a member of the Board of Study and Academic Council of Indrashil University and is the Member Secretary of the Institutional Biosafety Committee (IBSC) at the same University.

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