Principles and Techniques of Biochemistry and Molecular Biology

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Keith Wilson, John Walker
Cambridge University Press, 04.03.2010 - 744 Seiten
This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.
 

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Inhalt

Basic principles
1
Cell culture techniques
38
Centrifugation
73
Molecular biology bioinformatics and basic techniques
138
Recombinant DNA and genetic analysis
195
Immunochemical techniques
263
Protein structure purification characterisation
300
Mass spectrometric techniques
352
I Spectrophotometric techniques
477
II Structure and interactions
522
Radioisotope techniques
553
Principles of clinical biochemistry
625
Cell membrane receptors and cell signalling
660
Drug discovery and development
709
Index
736
Urheberrecht

Electrophoretic techniques
399

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Über den Autor (2010)

Keith Wilson is Professor Emeritus of Pharmacological Biochemistry and former Head of the Department of Biosciences, Dean of the Faculty of Natural Sciences, and Director of Research at the University of Hertfordshire.

John Walker is Professor Emeritus and former Head of the School of Life Sciences at the University of Hertfordshire.

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