Modulation of UNC-86 Activity During Caenorhabditis Elegans Neurogenesis

activate transcription activation domain alleles amino acid amino-terminal assays B-galactosidase Baumeister Caenorhabditis briggsae Caenorhabditis elegans cDNA Chalfie cloned coactivation conserved containing CS1 DNA data not shown DNA binding DNA-binding domain domain of UNC-86 domains of MEC-3 egg-laying EMSA encodes expression pattern Figure was modified Finney fosmid function Genes Dev His-tagged Hobert homeodomain protein homologs hybrid injected interaction of UNC-86 interaction with MEC-3 intron lacZ lacZ reporter gene LexA LIM domains LIM homeodomain protein lineage mec-3 expression mec-3 promoter MEC-3AD mechanosensation mechanosensory neurons mutant mutant animals nematode nucleotide OCA-B octamer motif oligonucleotide phenotype phosphorylation plasmid POU domain POU proteins POUHD POUSD protein interactions regulation reporter gene reporter gene activation residues RNAi Röckelein Röhrig Ruvkun ternary complex formation transcription factor transcriptional activation UNC-86 activity UNC-86 binding unc-86 cDNA unc-86 expressing unc-86 mutants UNC-86 POU domain UNC-86 protein UNC-86 variant UNC-86 with MEC-3 vivo wild-type wild-type UNC-86 yeast strain