GB/T 14701-2019 Translated English of Chinese Standard. (GBT 14701-2019, GB/T14701-2019, GBT14701-2019): Determination of Vitamin B2 in feedshttps://www.chinesestandard.net, 31.08.2019 - 14 Seiten This Standard specifies the fluorescence spectrophotometry and highperformance liquid chromatography for the determination of vitamin B2 (namely riboflavin C17H20N10) in feeds. Method 1 of this Standard is applicable to the determination of vitamin B2 in animal and plant feed materials, compound feed and concentrated feed; the quantitation-limit is 0.25 mg/kg. |
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100 mL brown accurate to 0.001 acetic acid 4.2.4 acetic acid solution acid solution 3.2.8 add 1 mL arithmetic mean B2 stock solution bromocresol green brown conical flask brown volumetric flask chromatogram of vitamin compound feed compound premix feed concentrated feed content of vitamin deionized water determination of vitamin determination values dilute to 100 disodium hydrogen phosphate EDTA feeds whose vitamin Figure A.1 filter fluorescence detector fluorescence intensity glacial acetic acid guaranteed reagent High-performance liquid chromatography Hydrochloric acid solution independent determination results intensity of test methanol milliliters mL brown conical mL brown volumetric mL of glacial mL of vitamin Mobile phase mol/L obtained under repeated potassium permanganate quantitation-limit refrigerator at 2°C repeated conditions riboflavin sodium dithionite sodium hydroxide solution solution contains spectrophotometry standard chromatogram standard stock solution standard working solution test solution test tube triethylamine vitamin B2 content Vitamin B2 standard Vitamin B2 stock vitamin premix feed water to dilute