An Introduction to Molecular BiotechnologyWritten by exceptionally experienced experts from one of the world's major biotech regions, the Heidelberg area, the authors come from academia, such well-known research centers as the German Cancer Research Center, and biotech companies. A very detailed introduction to the fundamentals in molecular and cell biology is followed by an overview of standard techniques applied in molecular biotechnology -- including chromatography and electrophoresis, cloning techniques, gene expression systems, immunological methods, labeling of proteins and in situ-techniques, microscopy and laser systems. The third part then focuses on the key topics of molecular biotechnology, ranging from functional genomics, proteomics and bioinformatics to drug targeting, recombinant antibodies, structural biology, gene therapy and knock mice. The whole is rounded off by a section on biotechnology in industry dealing with patenting issues, company foundation and market opportunities. On over 800 pages this book provides students and professionals in life sciences, pharmacy and biochemistry with all they need to know about molecular biotechnology. |
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Inhalt
Fundamentals of Cellular and Molecular Biology | 1 |
Structure and Function of Cellular Macromolecules | 7 |
Structure and Function of a Cell | 36 |
An Introduction to Molecular Biotechnology Edited by M Wink | 77 |
Distributing Proteins in the Cell Protein Sorting | 109 |
Diversity of Organisms | 121 |
Standard Methods in Molecular Biotechnology | 129 |
Peptide and Protein Analysis with Electrospray Tandem Mass | 151 |
ProteinProtein and ProteinDNA Interaction | 365 |
Bioinformatics | 387 |
Drug Research | 413 |
Drug Targeting and Prodrugs | 437 |
3 | 443 |
Molecular Diagnostics in Medicine | 455 |
Recombinant Antibodies and Phage Display | 479 |
Genetically Modified Mice Transgenic and Knockout | 511 |
Isolation of DNA and RNA | 165 |
Chromatography and Electrophoresis of Nucleic Acids | 171 |
Polymerase Chain Reaction PCR | 189 |
DNA Sequencing | 197 |
Cloning Procedures | 203 |
Expression of Recombinant Proteins | 223 |
The Patch Clamp Method | 239 |
Cell Cycle Analysis | 247 |
Techniques in Microscopy | 257 |
Laser Applications | 265 |
Key Topics | 271 |
Functional Genomics | 321 |
Modified DNA PNA and Applications in Medicine | 543 |
Plant Biotechnology | 557 |
Biocatalysis in the Chemical Industry | 583 |
Biotechnology in Industry | 613 |
Drug Approval in the European Union and United States | 647 |
The Emergence of a Biotechnology Industry | 659 |
The 101 of Founding a Biotech Company | 671 |
Marketing | 683 |
Glossary | 689 |
733 | |
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