An Introduction to Molecular Biotechnology

Cover
Michael Wink, Renate FitzRoy
John Wiley & Sons, 02.10.2006 - 768 Seiten
Written by exceptionally experienced experts from one of the world's major biotech regions, the Heidelberg area, the authors come from academia, such well-known research centers as the German Cancer Research Center, and biotech companies.
A very detailed introduction to the fundamentals in molecular and cell biology is followed by an overview of standard techniques applied in molecular biotechnology -- including chromatography and electrophoresis, cloning techniques, gene expression systems, immunological methods, labeling of proteins and in situ-techniques, microscopy and laser systems. The third part then focuses on the key topics of molecular biotechnology, ranging from functional genomics, proteomics and bioinformatics to drug targeting, recombinant antibodies, structural biology, gene therapy and knock mice. The whole is rounded off by a section on biotechnology in industry dealing with patenting issues, company foundation and market opportunities.
On over 800 pages this book provides students and professionals in life sciences, pharmacy and biochemistry with all they need to know about molecular biotechnology.
 

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Inhalt

Fundamentals of Cellular and Molecular Biology
1
Structure and Function of Cellular Macromolecules
7
Structure and Function of a Cell
36
An Introduction to Molecular Biotechnology Edited by M Wink
77
Distributing Proteins in the Cell Protein Sorting
109
Diversity of Organisms
121
Standard Methods in Molecular Biotechnology
129
Peptide and Protein Analysis with Electrospray Tandem Mass
151
ProteinProtein and ProteinDNA Interaction
365
Bioinformatics
387
Drug Research
413
Drug Targeting and Prodrugs
437
3
443
Molecular Diagnostics in Medicine
455
Recombinant Antibodies and Phage Display
479
Genetically Modified Mice Transgenic and Knockout
511

Isolation of DNA and RNA
165
Chromatography and Electrophoresis of Nucleic Acids
171
Polymerase Chain Reaction PCR
189
DNA Sequencing
197
Cloning Procedures
203
Expression of Recombinant Proteins
223
The Patch Clamp Method
239
Cell Cycle Analysis
247
Techniques in Microscopy
257
Laser Applications
265
Key Topics
271
Functional Genomics
321
Modified DNA PNA and Applications in Medicine
543
Plant Biotechnology
557
Biocatalysis in the Chemical Industry
583
Biotechnology in Industry
613
Drug Approval in the European Union and United States
647
The Emergence of a Biotechnology Industry
659
The 101 of Founding a Biotech Company
671
Marketing
683
Glossary
689
Subject Index
733
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Über den Autor (2006)

Professor Michael Wink studied Biology in Bonn and Braunschweig, Germany. After post-doctoral work in Cologne and Munich he took up a professorship at the University of Mainz, Germany. Since 1989 Michael Wink has been Professor of Pharmaceutical Biology at the University of Heideberg and was appointed Director of the Institute of Pharmacy and Molecular Biotechnology in 2002. His scientific interests focus on bioactive natural products, pharmacology & toxicology of natural products, and molecular evolution. Professor Wink is author of numerous scientific papers and pharmaceutical biology books.

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