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Monoclonal Antibodies:

A Practical Approach
Front Cover
Philip S. Shepherd, Christopher J. Dean
1 Review
Oxford University Press, 2000 - Medical - 479 pages
Monoclonal Antibodies: A Practical Approach covers the preparation, testing, derivation, and applications of monoclonal antibodies. New immunological techniques incorporating tried and tested methodologies are described, making the book of interest to established and inexperienced immunologists. Both the standard somatic hybridization technique and recombinant techniques, including the use of phage libraries, for the preparation of rodent and human monoclonal antibodies are described. Protocols for both the small and large scale production are detailed, as well as purification and labelling (with both radioisotopes and non-radioisotopes) methods. The applications of monoclonal antibodies in immunoblotting, enzyme linked immunoassays, immunofluorescence, and FACS analysis are all covered in detail. Finally protocols are given for the use of monoclonal antibodies in rheumatoid arthritis, tissue typing, detecting DNA modified during chemotherapy, and in the clinical analysis of transplantation samples for malignancy. This book will therefore be an invaluable laboratory companion to anyone using monoclonal antibodies in their research.
  

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Selected pages

Contents

Preparation of recombinant antibodies from immune rodent
25
Appendix
58
j Selection of antibodies from phage libraries of immunoglobulin
67
Proximity selections
78
ARM complexes for in vitro display and evolution of antibody
91
Ribosome display methodology
93
Troubleshooting
107
EBV transformation of B cells
114
Radiolabelling of monoclonal antibodies
207
Nonradioactive antibody probes
237
Immunogold probes for light and electron microscopy
247
Characterization of cellular antigens using monoclonal antibodies
265
Immunoassays
297
Immunoaffinity chromatography of macromolecules
319
Immunochemical detection of BrdUrd labelled nuclei for monitoring cell
341
Immunofluorescence microscopy
355

Cryopreservation of cells
122
Laboratory based methods for small scale production of monoclonal
125
Stirred cultures
131
Perfusion high cell densityl systems
137
Harvesting and concentration
143
Isolation and purification of monoclonal antibodies from tissue
149
Precipitation methods
156
Antibody production in plants
181
FACS analysis of clinical haematological samples in transplantation
371
Immunocytochemical staining of cells and tissues for diagnostic
391
Detection of chemically modified DNA in lymphocytes of patients undergoing
411
Monoclonal antibody therapy in organ transplantation
431
Monoclonal antibody therapy in rheumatoid arthritis
449
Al List of suppliers
463
index
469
Copyright

Other editions - View all

Monoclonal Antibodies: A Practical Approach
Monoclonal Antibodies: A Practical Approach
Philip S. Shepherd,Christopher J. Dean
No preview available - 2000

Common terms and phrases

acid adducts affinity agarose aliquots alkaline alkaline phosphatase analysis anti antigen approx assay avidin beads binding biotin biotinylated blot BrdUrd buffer pH cell lines Centrifuge chromatography clones coated column complex concentration conjugate culture supernatant cycle cytokine denaturation described detection dilution ELISA elution enzyme epitope Equipment and reagents ethanol fluorescence fraction fragments g/ml gene harvests human hybridoma immune immunoglobulin ImmunoL immunoprecipitation Incubate labelled antibody ligand lymphocytes lysis matrix medium membrane method mg/ml microscope microtitre molecules monoclonal antibody mouse PBS containing pellet phage pipette plant plate preparation primer procedure production protein Protocol purification radiolabelled radionuclide reaction reagents remove resuspend room temperature RT-PCR sample scFv SDS-PAGE secondary antibody sections selection serum signal slides sodium solution specific staining step sterile substrate supernatant technetium techniques tissue transfer transgenic Transplantation Tris-HCl tube vector vitro volume w/vl Wash

References to this book

From Google Scholar

Production and purification of ovine anti-tetanus antibody
El-Rashdy M Redwan, Ahmed Khalil, Zeinab Z EL-Dardiri - 2005 - Comparative Immunology, Microbiology and Infectious Diseases
Establishment and Characterization of Monoclonal Antibodies ...
Kazuo Ohnishi
Monoclonal antibodies: production, purification and enzymatic ...
Practical Immunology
Novel platform for minimizing cell loss on separation process ...
Youngho Kim, Su Hong, Sang Ho Lee, Kangsun Lee, Seok Yun, Yuri Kang, Kyeong-Kap Paek, Byeong ... - 2007 - Review of Scientific Instruments
All Scholar search results »

References from web pages

OUP: UK General Catalogue
Monoclonal Antibodies: A Practical Approach covers the preparation, testing, derivation, and applications of monoclonal antibodies. ...
www.oup.co.uk/ isbn/ 0-19-963722-9

Ann Intern Med -- 133 (11): 0
Monoclonal Antibodies: A Practical Approach. P Shepherd and C Dean, eds. 479 pages. New York: Oxford Univ Pr; 2000. $90.00. ISBN 0199637229. ...
www.annals.org/ cgi/ content/ full/ 133/ 11/ 0

MONOCLONAL ANTIBODIES : A PRACTICAL APPROACH (VOL 227) - Shepherd ...
MONOCLONAL ANTIBODIES : A PRACTICAL APPROACH (VOL 227) - Shepherd.
shepherd.comprar-livro.com.br/ livros/ 1019963722/

(WO/2007/067877) PROTOCOL FOR DETECTING PROTEIN IN A CULTURE ...
Monoclonal Antibodies: A Practical Approach. Oxford University Press, 2000; Coligan et al., eds. Current Protocols in Immunology, Vol. 1. ...
www.wipo.int/ pctdb/ en/ wo.jsp?wo=2007067877& IA=WO2007067877& DISPLAY=DESC

The American Journal of the Medical Sciences - Fulltext: Volume ...
Monoclonal antibodies: a practical approach. New York: Oxford University Press; 2000. p. 1-24. [Context Link]. 13. Funaro A, Horenstein AL, ...
www.amjmedsci.com/ pt/ re/ ajms/ fulltext.00000441-200207000-00004.htm;jsessionid=H1xMK5N8CLv57zDhLVc4pQKQdHQ1vg5PvKzrv4...

Identification of tumor-associated antigens for diagnosis and ...
(for technical details see: " Monoclonal Antibodies: A Practical Approach" by Philip Shepherd, Christopher Dean ISBN 0-19-963722-9 ; " Antibodies: A ...
www.freepatentsonline.com/ EP1762575.html

Oxford University Press:Monoclonal Antibodies-Academic exchanges
Monoclonal Antibodies: A Practical Approach covers the preparation, testing, derivation, and applications of monoclonal antibodies. ...
www.5zu.cn/ post/ 8200.html

(WO/2007/068449) MOLECULAR MARKERS FOR THE DIAGNOSIS AND TREATMENT ...
... können über verschiedene Standard verfahren hergestellt werden; vgl. beispielsweise „Monoclonal Antibodies: A Practical Approach" von Philip Shepherd, ...
www.wipo.int/ pctdb/ en/ wo.jsp?IA=WO2007068449& wo=2007068449& DISPLAY=DESC

About the author (2000)

Phil S. Shepherd is at The Guy's, King's College, and St Thomas's Hospitals, London.

Bibliographic information

QR code for Monoclonal Antibodies
TitleMonoclonal Antibodies: A Practical Approach
Issue 227 of The practical approach series
EditorsPhilip S. Shepherd, Christopher J. Dean
Editionillustrated
PublisherOxford University Press, 2000
ISBN0199637229, 9780199637225
Length479 pages
Subjects ›  › 

Medical / Immunology
Medical / Laboratory Medicine
Science / Life Sciences / Cell Biology
  
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