Principles and Techniques of Biochemistry and Molecular BiologyKeith Wilson, John Walker Cambridge University Press, 04.03.2010 - 744 Seiten This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained. |
Inhalt
Basic principles | 1 |
Cell culture techniques | 38 |
Centrifugation | 73 |
Microscopy | 100 |
Molecular biology bioinformatics and basic techniques | 138 |
Recombinant DNA and genetic analysis | 195 |
Immunochemical techniques | 263 |
Protein structure purification characterisation and function analysis | 300 |
Spectroscopic techniques I Spectrophotometric techniques | 477 |
Spectroscopic techniques II Structure and interactions | 522 |
Radioisotope techniques | 553 |
Enzymes | 581 |
Principles of clinical biochemistry | 625 |
Cell membrane receptors and cell signalling | 660 |
Drug discovery and development | 709 |
| 736 | |
Mass spectrometric techniques | 352 |
Electrophoretic techniques | 399 |
Chromatographic techniques | 433 |
c51635191 | 745 |
Andere Ausgaben - Alle anzeigen
Principles and Techniques of Biochemistry and Molecular Biology Keith Wilson,John Walker Keine Leseprobe verfügbar - 2010 |
Häufige Begriffe und Wortgruppen
activity agarose agonist allosteric allows amino acid analysis analyte antibody antigen assay atoms bacterial binding biochemical biological buffer cDNA cell culture cellular centrifugation chemical chromatography clinical cloning column complex concentration containing denatured detection detector determined DNA polymerase drug electron electrophoresis elution emission energy enzyme equation example fluorescence fluorophore fragments function gene genome glucose GPCRs gradient groups hydrophobic identified inhibitor interaction involved ionisation ions isotopes kinase labelled laboratory ligand light mass spectrometry measure medium membrane metabolism method microscope molecular mass molecules monoclonal antibodies mRNA nucleic acids particles pathway peptide phage plasmid plate polymerase primer probe produce protein proteomics purification range reaction reagents receptor residues result rotor sample Section separation sequence serum signal solution specific specimen spectrometry spectroscopy spectrum stationary phase strand structure studies substrate subunits synthesis techniques temperature tissue types vector wavelength