Guide to Yeast Genetics and Molecular Biology, Teil 1Christine Guthrie, Gerald R. Fink Academic Press, 1991 - 933 Seiten Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made "Saccharomyces cerevisiae" such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features* Basic Methods in Yeast Genetics* Physical and genetic mapping* Making and recovering mutants* Cloning and Recombinant DNA Methods* High-efficiency transformation* Preparation of yeast artificial chromosome vectors* Basic Methods of Cell Biology* Immunomicroscopy* Protein targeting assays* Biochemistry of Gene Expression* Vectors for regulated expression* Isolation of labeled and unlabeled DNA, RNA, and protein. |
Inhalt
JAMES HICKS | 21 |
JANE C Schneider | 25 |
Mapping Yeast Genes FRED SHERMAN | 38 |
Urheberrecht | |
48 weitere Abschnitte werden nicht angezeigt.
Andere Ausgaben - Alle anzeigen
Guide to Yeast Genetics and Molecular and Cell Biology: Guide to ..., Teil 1 Christine Guthrie,Gerald R. Fink Eingeschränkte Leseprobe - 2004 |
Guide to Yeast Genetics and Molecular Biology, Teil 1 Christine Guthrie,Gerald R. Fink Keine Leseprobe verfügbar - 1991 |
Häufige Begriffe und Wortgruppen
a-factor Acad acetate acid agar allele analysis antibody assay BamHI Biol buffer Cell Cambridge centrifugation centromere cerevisiae chromosome cloned gene Cold Spring Harbor coli colonies complementation containing culture described diploid distal E. W. Jones EcoRI EDTA electrophoresis enzyme ethanol extract filter frequency G. R. Fink galactose genetic genome haploid Hieter HindIII HIS3 homologous hybridization Incubate insert integration isolated LEU2 ligation locus LYS2 mapping MATa mating type mating-type medium meiosis meiotic met2 method mitochondrial mitotic molecular mRNA mutagenesis mutagenized mutant Natl pellet phenotype pheromone plasmid prepared Proc procedure protein protocol recombination replica plated replication restriction Resuspend ribosomal Saccharomyces Saccharomyces cerevisiae sample screening segment segregation sequences shuttle vector solution spheroplasts spores sporulation tetrads tion transcription transformation transposition transposon tube Ty elements URA3 vector volume wild-type YCF3 yeast yeast genes yeast strain YEPD µg/ml