An Introduction to Molecular BiotechnologyMichael Wink John Wiley & Sons, 02.10.2006 - 768 Seiten Written by exceptionally experienced experts from one of the world's major biotech regions, the Heidelberg area, the authors come from academia, such well-known research centers as the German Cancer Research Center, and biotech companies. A very detailed introduction to the fundamentals in molecular and cell biology is followed by an overview of standard techniques applied in molecular biotechnology -- including chromatography and electrophoresis, cloning techniques, gene expression systems, immunological methods, labeling of proteins and in situ-techniques, microscopy and laser systems. The third part then focuses on the key topics of molecular biotechnology, ranging from functional genomics, proteomics and bioinformatics to drug targeting, recombinant antibodies, structural biology, gene therapy and knock mice. The whole is rounded off by a section on biotechnology in industry dealing with patenting issues, company foundation and market opportunities. On over 800 pages this book provides students and professionals in life sciences, pharmacy and biochemistry with all they need to know about molecular biotechnology. |
Inhalt
Fundamentals of Cellular and Molecular Biology | 1 |
Structure and Function of Cellular Macromolecules | 7 |
Structure and Function of a Cell | 39 |
An Introduction to Molecular Biotechnology Edited by M Wink | 77 |
Distributing Proteins in the Cell Protein Sorting | 109 |
Diversity of Organisms | 121 |
Standard Methods in Molecular Biotechnology | 129 |
Peptide and Protein Analysis with Electrospray Tandem Mass | 151 |
ProteinProtein and ProteinDNA Interaction | 365 |
Bioinformatics | 387 |
Drug Research | 413 |
Drug Targeting and Prodrugs | 437 |
Recombinant Antibodies and Phage Display | 479 |
Genetically Modified Mice Transgenic and Knockout | 511 |
Strategies and Vectors | 523 |
Modified DNA PNA and Applications in Medicine | 543 |
Isolation of DNA and RNA | 165 |
Chromatography and Electrophoresis of Nucleic Acids | 171 |
Polymerase Chain Reaction PCR | 189 |
DNA Sequencing | 197 |
Cloning Procedures | 203 |
Expression of Recombinant Proteins | 223 |
The Patch Clamp Method | 239 |
Cell Cycle Analysis | 247 |
Techniques in Microscopy | 257 |
Laser Applications | 265 |
Key Topics | 271 |
Plant Biotechnology | 557 |
Biocatalysis in the Chemical Industry | 583 |
Biotechnology in Industry | 613 |
Drug Approval in the European Union and United States | 647 |
The Emergence of a Biotechnology Industry | 659 |
The 101 of Founding a Biotech Company | 671 |
Marketing | 683 |
Glossary | 689 |
733 | |
Andere Ausgaben - Alle anzeigen
Häufige Begriffe und Wortgruppen
activity amino acids analysis analyzed antibody fragments antigen application assays bacteria binding biological biotech biotechnology bispecific bonds cancer cDNA cell cycle chain Chapter chemical chromosomes clones coli companies complex compounds contain cytoplasmic detection diabody disease DNA fragments DNA polymerase DNA sequence domains drug electrophoresis eukaryotic example factor fluorescence function fusion protein gene expression gene therapy Genentech genetic genome human genome hybridization important infection interactions kinase laser membrane metabolic methods microarray microtubules Molecular Biotechnology molecules mouse mRNA mutations nucleic acids nucleotides oligonucleotide organisms patent peptide phage pharmaceutical plant plasmid polymerase possible primer prodrug production quence reaction receptor recombinant protein replication restriction enzymes ribosomes scFv selection marker signal specific stable strand structure substances synthesis target teins therapeutic tion tissue transcription transformation transgenic tRNA tumor vectors viral virus viruses vitro Weinheim Wiley-VCH yeast